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GrainGenes Sequence Report: BE493641

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Sequence
BE493641
Contig
Ta.4358.1.S1_s_at
Ta.4358.1.S1_x_at
NSFT03P2_Contig8575
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Germplasm
T. monococcum ssp. monococcum DV92
Species
Triticum monococcum
Cultivar
DV92
Clone Library
Triticum monococcum vegetative apex cDNA library
Tissue
Vegetative shoot apex
Developmental Stage
Three weeks-old plants
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0568_D11_G22ZE Triticum monococcum vegetative apex cDNA library Triticum monococcum cDNA clone WHE0568_D11_G22, mRNA sequence.
Other Name
WHE0568_D11_G22ZE  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli XLOLR
DNA Library
TM011XXX
Clone
WHE0568_D11_G22
Probe
[Wcl1018ct1900cn2502]
Remark
DB_xref: taxon:4568
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene T3 primer.
Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ggcacgagcaaatcaccacaggtccgtccagcaggaggtggggagggaga
agatggaccatcaccacccgccgccgctcccgcagcagcacggcgaccac
taccggccgctggtgctctctccgcagcccgaccatgcccacgcccttca
gtaccagcagccgccgcagcagcaagcgacgccgccgctgcaacaccacc
acccctcgctcgcgtcccacttccacctcctccacttggtgacaagatta
ggtgatgcgattgcaacaggcacaagggatcaagcttttgatgcattggt
tgaagaattgaccagtcaattcgccaggagccaacagttattgaactcca
tttcaggaacactaagctcgaaatctgttacggttgaaggacaaatgcaa
agtttggaagaaacccgtcagctgcttgatcaaaggaaggatttgattgc
gaaatacaaaagctcagtggaagacctccttaaggggaatccaacaagat
aaaaaaggctctgacagtcagaccctcagaacattaattatgatggagcc
cgttgccaccaccgcctgaaataaattcctcatcgatgcgtcttatcatg
taaacatgct

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