GrainGenes Sequence Report: BE494575

Sequence

BE494575

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

BE494575

DB Remark

Locus Source: Secale cereale (rye)

Keyword

EST

Germplasm

Blanco

Species

Secale cereale

Cultivar

Blanco

Chromosome

4AS

4BL

4DL

Clone Library

Secale cereale anther cDNA library

Tissue

Anther

Developmental Stage

Adult plant before anthesis

Data Source

genbank	Release 135, Apr 15 2003
genbank	Downloaded 2008-2009

Title

WHE1255_F10_K19ZS Secale cereale anther cDNA library Secale cereale cDNA clone WHE1255_F10_K19, mRNA sequence.

Other Name

WHE1255_F10_K19ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

SC024E1X

Clone

WHE1255_F10_K19

Probe

WHE1255_F10_K19

Remark

DB_xref: taxon:4550

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA Homology

Ta.9130.2.S1_s_at	222	WHE2AFFY
Ta.9130.2.S1_at	222	WHE2AFFY
Ta.9130.2.S1_at	119	WHE2AFFY

DNA

cactagacaagctgctgttgcgaggtttgaacatcacaattctgggttcc
aatgatttctattcgtacaggaagcagattgaagcacgtggtctgcctct
aactccagaggtattgctttcattgcctccattttgctcaattacgttta
acccagaagttgctaatggaactggagaaatatgcaaacctgaagttgca
aaaactggacttgggtcatcagctgccatgactgcatcagttgtcgctgc
tcttcttcattatcttggagctgttaacctttcgtgttcgggtcaatcat
ccagtgactctgtaactggacgagattttgactttgtgcatgctattgct
caaagtgcacactgtatagcacaagggaaaatcggcagtggctttgatgt
tagtgccgctgtctatgggagtcagcgctacataaggttctctccggaaa
tcctctcctcagctcaggttataggcgggagttgcttgccagatgtagta
gcagatattgtta


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