GrainGenes Sequence Report: BE495292

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Sequence

BE495292

Contig

NSFT03P2_Contig11416

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

wEST map position

BE495292

DB Remark

Locus Source: Secale cereale (rye)

Keyword

EST

Germplasm

Blanco

Species

Secale cereale

Cultivar

Blanco

Chromosome

1AS

1BS

1DS

Clone Library

Secale cereale anther cDNA library

Tissue

Anther

Developmental Stage

Adult plant before anthesis

Data Source

genbank	Release 135, Apr 15 2003
genbank	Downloaded 2008-2009

Title

WHE1268_F11_K22ZS Secale cereale anther cDNA library Secale cereale cDNA clone WHE1268_F11_K22, mRNA sequence.

Other Name

WHE1268_F11_K22ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

SC024E1X

Clone

WHE1268_F11_K22

Probe

WHE1268_F11_K22

BE495292

Remark

DB_xref: taxon:4550

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

gacccccaactccgtcgacggctcgatcgatctcggcaaaccgcagcgga
tctcgccggcagcgaaggcgcggggggagctatggacgcggagctcctgg
agctgcagcggcagctggaggcggcgcagagcgcgcggtcgagcgtgcgg
ctgtcggagcgcaacgtggtggagctcgtgcagaagctgcaggagcgcgg
cctcatcgacttcgagctcctccacaccgtctccggcaaggagtacatca
catccgaccatttgaagcatgagattaaggtggagatcaagaagcgaggg
cgcgcatcactagtcgatttatcagataccctgggggtggatctttacca
tattgagaggcaggcacaaaaggtcgtcacggaggatccggccctgatgt
tgatcaacggggagatcatgtcacagtcttactgggacactgtgacggaa
gagataaatgagaagttgcaagagcgcagccagattgccttggctgagat
tgctgcacagttacacattggctcagagctggtcctcaatattcttgagc
ct


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