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GrainGenes Sequence Report: NSFT03P2_Contig7274

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Sequence
BE495400
Contig
NSFT03P2_Contig7274
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC4938
wEST map position
BE495400
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Germplasm
Blanco
Species
Secale cereale
Cultivar
Blanco
Chromosome
7AL
7BL
7DL
Clone Library
Secale cereale anther cDNA library
Tissue
Anther
Developmental Stage
Adult plant before anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankDownloaded 2008-2009
Title
WHE1270_E02_J04ZS Secale cereale anther cDNA library Secale cereale cDNA clone WHE1270_E02_J04, mRNA sequence.
Other Name
WHE1270_E02_J04ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
SC024E1X
Clone
WHE1270_E02_J04
Probe
WHE1270_E02_J04
Remark
DB_xref: taxon:4550
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.9851.1.S1_at52WHE2AFFY
[ Show all 6 ]
DNA
cgcctacggagggtaacatcagcgacaagaacccttttccgccattgcac
ctagcaaccaccattaatttgccgccgagaaatcctgcacggcattgatc
gatgcgctgcacatgatctctgccaggttcgccgccgcgctgagaaacga
gaaggtctgggtgatgaacgtcgtcaacgtggacgcgccggacacgctgc
ccatcatcttcgaccgcgggctgttcggcatgtaccatgactggtgcgag
tccttcagcacctacccgaggacctacgaccttctgcacgccgatcacct
cttctcaaagataaaggacaggtgcgccgtgctgccggtcatcgtcgagg
tggacaggatcgtgaggccgggagggagcgtcatcgtgcgcgacgactcc
ggcgtcgtcggcgaagtggagaagctcttgaggtcgcttcactgggacgt
gaggctgaccttctccaagaacaacgaaggggtgctgttcgccgagaaat
cggattggcggccggagctggtcgccgagccagcttaaaccaagccctct
ccattttc

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