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GrainGenes Sequence Report: BE496889

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Sequence	BE496889
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC324768
wEST map position	BE496889
NCBI UniGene	Ta.7310
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001049932.1 Os03g0314200 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4AS 4BL 4DL
Clone Library	Wheat heat-stressed seedling cDNA library
Tissue	Leaf
Developmental Stage	Two-week old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0761_A10_B19ZS Wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0761_A10_B19, mRNA sequence.
Other Name	WHE0761_A10_B19ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA015E1X
Clone	WHE0761_A10_B19
Probe	WHE0761_A10_B19
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	cccctcctcctccgattcgcgtttggatcccagcgccaaacttggcggat gctgtacaaatggcggaggcgctcgcgtccgcgggtgggctcgccatggc ggcctcgacctccttcacgcccggccagctttctgcttttctgggatttc tttgggttcttgcggcctgcgtgtatgctgaggttctctttcatacgaag aatgctgcttgacttaaaacacactcggacatcaattgatcacacatgga tagtagctcaaacaaaggagaggatcaaacaatgttactggaggagggtg gtccagtagctggtgcaaagccagtttatgcttctttcacatcacagatg ctcacacttttctgtatggacgagacacttcttctggagcatcgattgac actgagggcaacatccgagcttggaggccatctactttatttctacatat gtgaccgcacaaatatattcggagagtctgaaaagaattacagccgagat ctc

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