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GrainGenes Sequence Report: BE497600

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Sequence
BE497600
Contig
NSFT03P2_Contig17408
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC277181
wEST map position
BE497600
NCBI UniGene
Ta.50569
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'EREBP/AP2 type transcription factor'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL
1BL
1DL
2BL
Clone Library
Wheat heat-stressed seedling cDNA library
Tissue
Leaf
Developmental Stage
Two-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0754_D07_H14ZS Wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0754_D07_H14, mRNA sequence.
Other Name
WHE0754_D07_H14ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA015E1X
Clone
WHE0754_D07_H14
Probe
[Wcl651ct1472cn2017]
WHE0754_D07_H14
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.45961.1.S1_at54WHE2AFFY
DNA
acacaatgtggattccgtggtgtaaggcaacgtacttgggggaagtgggt
tgctgaaattcgggagccaaatcgggtgagcaggctctggttgggaacgt
tccccactgctgaggatgctgcccgtgcttatgacgaggcagcaagagca
atgtatggcgccctggctcgtaccaacttccctgcgcatcctgcacaagc
tcctgctgtggctctaccagcggcaattgaaggtgttgtacgtggtgctt
cagcatcatgcgagtctactacaacatcag

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