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GrainGenes Sequence Report: BE498327

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Sequence	BE498327
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	BE498327
DB Remark	Locus Source: Triticum aestivum (bread wheat)
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	5BL 5DL
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0952_H05_O10ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0952_H05_O10, mRNA sequence.
Other Name	WHE0952_H05_O10ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA019E1X
Clone	WHE0952_H05_O10
Probe	BE498327 WHE0952_H05_O10
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	gcgtcgacgacttcaccaacggcctccgcccggatccccagcccgacgac tggctttccaaggcccgcgggttcctctacgagatctggaccttacgcag ccggagcagcatcgccgcccagatcgccgatctcaagcagcgggcgcagc atgtgggtgaccgccgcgtcaggtatggcgtccgcgacccgaatcccggc accgggaaaaatggtttgggtggatataccattgctgagcaccaggagat aacccgtcggcttatcggcatcaaggagccagttggggtggacatagcga agcttgaggagtggattagatctgaggagttgaagctgggtgttctatcc atatacggattcggcggtgtgggaaaaaccaccgccgccatggcactgta ccggagttgcggggttcatttcgagtgccgggcaatggtcaccgtgtctc atcacactgaccctgatgtagtcctctgcgacatactgaggcaagtcaag at

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