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GrainGenes Sequence Report: NSFT03P2_Contig7840

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Sequence
BE498794
Contig
Ta.10258.1.A1_at
NSFT03P2_Contig7840
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC292482
wEST map position
BE498794
NCBI UniGene
Ta.10258
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001060925.1 Os08g0130900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AS
5DS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0966_G09_N18ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0966_G09_N18, mRNA sequence.
Other Name
WHE0966_G09_N18ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE0966_G09_N18
Probe
WHE0966_G09_N18
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.10258.1.A1_at910WHE2AFFY
Ta.10258.1.A1_at444WHE2AFFY
Ta.7473.1.S1_at68WHE2AFFY
TaAffx.106257.1.S1_at66WHE2AFFY
DNA
cgtgtcccgtcacgcaaatcagtcactaaggctgttcaagatggaggatg
tgagcatgggcatgtgggtcgaagattacaacactaccaccatcaccgcc
ccggtgcagtacattcacagctggaagttctgccagtacgggtgcgtgga
caactacttcacggcgcactaccagtcccccaggcagatgctgtgcctct
gggacaaactgtcgttaggccgcgcgcagtgctgcaactacagataacac
gacgctcatccatggctgcttcaccagagcaactgatggcttaactaggc
ggcatccgtcagtcccactgccgtagtgtcattttagaagaacaccaggg
accaggccagcatgactgacacccaccagaccgggtgtccatcaccctta
actcgagttctctacatctgctctatcgactgacagcggcggagactatg
gacatcaccatt

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