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GrainGenes Sequence Report: BE499968

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Sequence
BE499968
Contig
Ta.1197.1.S1_at
Ta.1197.1.S1_x_at
NSFT03P2_Contig15309
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC277176
wEST map position
BE499968
NCBI UniGene
Ta.1197
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Alpha 1,4-glucan phosphorylase'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AL
3BL
3DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0976_B05_C10ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0976_B05_C10, mRNA sequence.
Other Name
WHE0976_B05_C10ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE0976_B05_C10
Probe
WHE0976_B05_C10
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.1197.1.S1_x_at963WHE2AFFY
Ta.1197.1.S1_at963WHE2AFFY
Ta.27525.1.S1_at194WHE2AFFY
Ta.1279.1.S1_at92WHE2AFFY
DNA
gtgttggatgtgactggtgttacaattgatccaaatagccttttcgatat
acaaattaaacgcatccacgaatacaagagacagctgttgaacattttgg
gagctgtgtacagatacaagaagttaaaggaaatgagcgcagaagagagg
aagaaggttacaccacgcactgtcatggtaggagggaaagcatttgcaac
atacaccaatgctaaaagaatagtgaaattggtaaatgatgttggtgctg
tggtgaacaatgatgctgacgtcaaccaatatctgaaggtggtgttcatt
ccaaactacaacgtatcagtggctgaagtgctcattcctggcagtgaact
gtcacagcacatcagtactgcaggcatggaagcaagtggaacaagtaaca
tgaagttctctctgaatggctgtgttatcattggaactctcgatggagcc
aatgttgaaatcagagaagaagtagggcaagacaacttcttccttttcgg
tgccaaagcagatcagattgctggtctg

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