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GrainGenes Sequence Report: BE517737

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Sequence
BE517737
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC355538
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0802_D07_G14ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0802_D07_G14, mRNA sequence.
Other Name
WHE0802_D07_G14ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA016E1X
Clone
WHE0802_D07_G14
Probe
BE517737
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
caagctgcgagccatccaggaggtggatggcaccaccgtcgccgtcgccg
ccgccgccgcgagtgtgcgtgaccggcggcggcgggttcatcgcctcgtg
gctcgtcaagctgctcctctcccggggctacgccgtccacgccacgctcc
gcgacccgagtgatccgaagaacgcgcatctgatgcagctggacggcgcg
gcggagagcctgagcttgttcaaggccgacgtgctcgaccgcgccgcgct
ggcc

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