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GrainGenes Sequence Report: BE517946

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Sequence	BE517946
Contig	Ta.28305.1.S1_a_at NSFT03P2_Contig10135
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC339177
wEST map position	BE517946
NCBI UniGene	Ta.31965
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001059634.1 Os07g0479300 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	2AL 6BS
Clone Library	Wheat vernalized crown cDNA library
Tissue	Crown tissue of seedling
Developmental Stage	Five-week old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0804_A01_B02ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0804_A01_B02, mRNA sequence.
Other Name	WHE0804_A01_B02ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA016E1X
Clone	WHE0804_A01_B02
Probe	WHE0804_A01_B02
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.28305.1.S1_a_at 1084 WHE2AFFY Ta.13505.1.S1_at 823 WHE2AFFY Ta.13505.1.S1_at 157 WHE2AFFY Ta.25362.1.A1_at 80 WHE2AFFY Ta.25362.1.A1_at 58 WHE2AFFY
DNA	tgaaggttttgcccttggtaacggacttacagatccagcgatacagtaca aggcatacaccgattatgcattggatatgggtttaatcacacagtcagaa tttaccaagattaataaaatagttcctgcttgtgaatttgctgtcaagct gtgcggtacctctggcactgtatcttgctttgctgcctattttgtttgca atacaatattcagttccatcaggttgatactcgggaacaaaaattattat gacgtcaggaagccatgcgtggggagcctatgctatgatttttctaactt ggagaagtttctcaatctgaaatctgtcagacagagcctaggcgttggag acatagagtttgtttcctgcagcccgaccgtctatcaggctatgctctta gattggatgaggaaccttgaagttgggatacctgaactccttgagaacga catcaaagtgctgatttatgctggagagtatgatctcatatgcaactggc tagatgggtagactctatggaatggtctggaaagaaagccttcgtgtcct cggtggagaaacccttcacggttgatggaaaagaagccggcattctaaaa agccacggccctttgagttttttg

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