GrainGenes Sequence Report: BE518101

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Sequence

BE518101

Contig

Ta.27117.2.S1_a_at

Ta.27117.6.S1_x_at

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

EST

Germplasm

Chinese Spring

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat vernalized crown cDNA library

Tissue

Crown tissue of seedling

Developmental Stage

Five-week old seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0810_H05_O10ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0810_H05_O10, mRNA sequence.

Other Name

WHE0810_H05_O10ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

TA016E1X

Clone

WHE0810_H05_O10

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

gtgattcctggcgtgtttgctgctatggctgtggaagctgtttaacctgt
gttatgtccaatttagctgtttaattctagtctggttatgt


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