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GrainGenes Sequence Report: BE518282

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Sequence
BE518282
Tracefile
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wEST map position
BE518282
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5BL
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0808_D10_H20ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0808_D10_H20, mRNA sequence.
Other Name
WHE0808_D10_H20ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA016E1X
Clone
WHE0808_D10_H20
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.10597.1.S1_at389WHE2AFFY
[ Show all 6 ]
DNA
gcttttgcaggcttacaatgatggatttgaagaccatggaacagtcctcg
aatttgttgcatcataaattgttggaactctggaataaacagaaagatgt
gaatggggaggcatacctgcctgcgatatacaccaacccaaagcttttga
gcgttgtacaaaagaagtacttgacaggtttacttcatgatagtgtggga
ttttgttcggtcagaatcatcaggtacttactcacttaatttccgaatat
accatttatttgttgaatgtattggttcgacttggtgatacatcaaagct
tctctttacctgaaacgacttaagggtgtattaaggtagtccaatgcatg

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