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GrainGenes Sequence Report: BE518440

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Sequence
BE518440
Contig
Ta.7532.2.S1_at
NSFT03P2_Contig10067
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC343222
wEST map position
BE518440
NCBI UniGene
Ta.54577
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'GTP-binding protein'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2AS
2BS
2DS
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0815_B04_D07ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0815_B04_D07, mRNA sequence.
Other Name
WHE0815_B04_D07ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA016E1X
Clone
WHE0815_B04_D07
Probe
WHE0815_B04_D07
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.7532.2.S1_at989WHE2AFFY
Ta.7532.2.S1_at541WHE2AFFY
DNA
atatcatggcaggttttgtactttcaagtgttgctaatcctattggtgct
ttcactgaatttaatgcccaactgcagattctagagttgctcgataatgc
tattttcactgctggttacaaggcagtgttacacatccactctgttgtcg
aggagtgtgagattgttgatctcatagaggaaattgacatgaagaaagca
aaagtaactgacccaaagaaaaagaagagcaagaggaagcctctttttgt
gaagaatggtgcagttgtagtttgccgcgtccaggtgactaatttgatat
gcatagagaagttctccgacttccctcagcttggaaggtttactctacga
actgaagggaagacaatagctgtaggcaaggttgttgatgtgcctccagt
tggcaggtcaacgttttcagcctaagccagttttgacaggatgatgggag
aatgaagtggcgctgtcttgaaatagtttttggaagcagcagtgagatcg
atttttccagccaggctccatgagtcacttagcattaatttattgctg

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