GrainGenes Sequence Report: BE585767
Sequence
BE585767
Contig
Ta.10307.1.A1_at NSFT03P2_Contig9706
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC288140
wEST map position
BE585767
NCBI UniGene
Ta.10307
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001065664.1 Os11g0132600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
4AL 4BS 4DS
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
Est#1SP6_G11_g11_084 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#1SP6_G11_g11_084, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#1SP6_G11_g11_084
Probe
Est#1SP6_G11_g11_084
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SP6. Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA Homology
Ta.10307.1.A1_at 690 WHE2AFFY Ta.10307.1.A1_at 371 WHE2AFFY Ta.10307.1.A1_at 430 WHE2AFFY Ta.4199.1.S1_a_at 56 WHE2AFFY
DNA
acgggggaaccttttcattcattttgatatatgcttgtggaacgtcaaca
agtagtgtcatggataccaggaccgatcacatgaggcctgataggcaaat
accagaaaaacaaacctatatttatttatctatatatccaatgaaccagg
gaagacaacttcacatttcgcttgctttctggaatccatgcaccagacct
gatcgatcaatgcctcgtgtagatcatcttgttttccacctcaccattgc
ttcttcacatgtcagctactagctcgccgtactcatccagagccagatca
tatgaagtgaaagagtacggaaccagcatggcctgcatctcttctgcagc
actggctagaccactttttaccggcttaaccttaagtgggtcatcgaacg
agttctcatccaacgaatagttggatgtgagaacggtgacagctgatctt
gacgtatcgacaccagcttccagtccggttgcagataatgtgaggttcac
agtattcggcccaaagttcgcaatctttatcctcaggaagatatcctcat
tgtctcgccaggtaattgcagatgctgccaatgaaccagagtagctggaa
ttgagtctcactggatggatcacagcaccacttgattcaccgataaa
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE585767
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||