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GrainGenes Sequence Report: BE585773

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Sequence
BE585773
Contig
Ta.7263.1.S1_a_at
Ta.7263.3.S1_a_at
NSFT03P2_Contig8256
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC284946
wEST map position
BE585773
NCBI UniGene
Ta.7263
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_173855.1 myosin-related [Arabidopsis thaliana]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Chromosome
6AL
6BL
6DL
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
Est#1SP6_H10_h10_080 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#1SP6_H10_h10_080, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#1SP6_H10_h10_080
Probe
Est#1SP6_H10_h10_080
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SP6.
Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA Homology
Ta.7263.1.S1_a_at1233WHE2AFFY
Ta.7263.1.S1_a_at82WHE2AFFY
Ta.7263.3.S1_a_at210WHE2AFFY
Ta.7263.3.S1_a_at202WHE2AFFY
Ta.7263.3.S1_a_at80WHE2AFFY
DNA
gcggggaacagctcaaggaggtttgcagaaacattgattcaaatcgtagg
gagttggaatcaaaaagcagtgaactgcttgaggtaacagtcagtatgga
gcgaatgattcagcggtttggatatcttgctggaaaagatgcgctggaag
ataataaacctgcaagcacacaaacactcttaccaaagctggaaaaacta
ataatcgcctcaagtatggaatctgggaatgccaagtctgtaaaacagga
gttgggatcaaagttgcaggccagggaaaagacagttgatgagttatcag
caaaagttaagatgcttgaggacttgtatcattctcagcttgtgcagcca
gaggttagtaaagacagagcatttgacgcatcttcctcggcaattggttc
agacatatccgagattgaagatttggggccaatggggaaggcatcggttt
catctgtccctactgctgcacatgccagagtaatgcggaagggttcatcc
gaccatcttgttcttaatatgggctcagagtctgaacgcctaattgctgc
acatgattcggatgacaaagggcgtattaagtcattgcatacatctggct
tgattccagcacaaggaaagcacatcgctgacagacttgatggcatctgg
gtctcagggagccaaattctgatgaaccgaccgcgg

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