GrainGenes Sequence Report: NSFT03P2_Contig4932
Sequence
BE585823
Contig
TaAffx.83824.1.S1_at NSFT03P2_Contig4932
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
Est#2sp6_B08_b8_061 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#2sp6_B08_b8_061, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#2sp6_B08_b8_061
Probe
[Wcl548ct1342cn1858]
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SP6. Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
gcggggatagccaagctaacgagcagtagcacatcgatcagcaaacgggc
gtccggcaggcactcaacacaaaacacaaatatgcgttcagctcgcgacc
tccgcctcgcggtctcggccctcctcctcctcctcctgctttcattcggc
gtcctggcggcaagagcccagcatgaaatcgacgatgagtcagagttcaa
ctacatccgcgggtccaagaacgggccggagaactggggcaagatcaagg
aggagtgggcgacgtgcggtaccgggcagatgcagtcgcctatcgacctc
tccgaccaccgtacctcacgggcacccaatctcggctacctcaaccactc
ctacctccctgtagaggcctgcatcgagaaccgcggccacgacatcatgg
acaagttcaacggcgacgccgggagcctgtggatcaacggggccgtgtgc
cacctgag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig4932
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||