GrainGenes Sequence Report: BE585881
Sequence
BE585881
Contig
TaAffx.107507.1.S1_at TaAffx.107507.2.S1_s_at NSFT03P2_Contig14206
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC350857
NCBI UniGene
Ta.62203
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001055558.1 Os05g0416400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
Est#3T7_H11_h11_092 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#3T7_H11_h11_092, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#3T7_H11_h11_092
Probe
MAG2933
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7. Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
gcgggggggccatcgccttagcagtttatgcttcttcgcgggtactactc
ctgactcctgttatatatatgtgacagactgacagggttgtgtggtcata
tggagtttggaaggaaatcttgggtggctgcagtcctctgatccttctca
ttctggaggcgtattcagtacaaaccaatattctggttggtgattgtcag
catggagatggcaagtagcgccaagtggtttgctgtggctcttgttttca
tcactgtgatcctactcagggtcataagaggaaggaggatggccgctgct
ccagccagtgagaaaccacctccacctgtggtgaatggttttgctttact
aggacttctacctaggcttcttacaatggaccttcgaactaagataaatt
gcctgcacgataagtatggcagtgtgttcacagttagtccttttggacta
actaatgtaacctttctgatcggcccggaggtccaatcccatttctttca
agggttggagtcagagattaaccatggcaatcttcttgagttccttgtgc
cgatgtttggacaagagataggtcaaggtgtcgatgcagccactcggact
gagcagtcccgcttttatctcgatgcattaaagcaatccaagttgaggag
acatcttgatccaatgcttcaagaggtcgagagctactttggcaaatggg
gcct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE585881
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||