GrainGenes Sequence Report: BE585926
Sequence
BE585926
Contig
Ta.28742.2.S1_x_at NSFT03P2_Contig18462
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC330268
NCBI UniGene
Ta.36473
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_181192.1 LOS2 (Low expression of osmotically responsive genes 1); phosphopyruvate hydratase [Arabidopsis thaliana]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
Est#4pT7_E03_e3_019 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#4pT7_E03_e3_019, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#4pT7_E03_e3_019
Probe
[Wcl102ct509cn822] {SpCl-193} BE585926
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7. Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
gcggggacccgtacagcgaacccgcacccctctcgctctccgctccatct
ggtccgattcgattcgattcgatctcggcttcctctccaagccacgcgag
accagccatggcggcgacgatccagtccgtgaaggcccgccagatcttcg
acagccgcggcaaccccaccgtcgaggttgatgtgtgctgctcagatgga
accttcgccagggccgccgttcccagcggtgcatcaactggtgtctatga
agctttggagctgagggacggtggatctgactacttgggcaagggtgttt
ccaaggctgttaacaatgtgaactccattattgcacccgctttggtcggc
aaggaccctaccgctcaaactgagctcgacaactttatggttcatcaggt
agatggaaccaagaatgagaggggttggtgcaagcgaaatcttgttgctg
atgcatttctggctgtatcactagctgttga
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE585926
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||