GrainGenes Sequence Report: BE586128
Sequence
BE586128
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC299325
NCBI UniGene
Ta.43528
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_187625.2 RECA3 (RECA HOMOLOG 3); DNA binding / DNA-dependent ATPase [Arabidopsis thaliana]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
KSU wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
Est#9SP6_H04_h4_032 KSU wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone Est#9SP6_H04_h4_032, mRNA sequence.
Strain
lab_host E. coli JM109
Clone
Est#9SP6_H04_h4_032
Probe
MAG2963
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: John Fellers; US Department of Agriculture, Agriculture Research Service, Plant; Science and Entomology Unit; Dept. of Plant Pathology, 4006 Throckmorton Hall, Kansas State; University, Manhattan, KS 66506, USA; Tel: 785-532-2367; Fax: 785-532-6167; Email: jpf@alfalfa.ksu.edu; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: SP6. Note: Vector: pGEM-T easy; Site_1: SacII; Site_2: SpeI; Plants were grown in the greenhouse. Spikes were sprayed with Fusarium graminearum (at what stage). Total RNA, and poly(A) RNA were prepared from infected spikes. cDNA was prepared using the SmartTM PCR cDNA synthesis kit from Clonetech. cDNA was cloned into the pGEM-T easy vector from Promega.
DNA
gcggggaaaatgctttcaacacttcatttgcagaagaagttggtgtggac
attgacaaactcctaatagctcaacctgattctgctgaaaattcattaag
catagtgaacactcttgttggtggatccattgatgttgttgttgtggata
gtgtggcagcactgattcccaaatgtgaacttgaaggtgaaatatacatg
aaatttaaagacgcacaatctcgtttaatgactcgggcacttcgaaaaat
tcaatacacattaagtcgctctgaaacacttattatttttgtgaatcagg
ttagaacaaagcgtagctcagatccatcttctgggctctacaaggaggta
acctgtggtggcaacgcgttagggttctattcagcaatcagaatgaggac
atcgagaaggaaattgcactattgcaaagaagaggccactggcatcagta
tagcagtgcagatcatcaagaacaaattagtaccagcagccctgaaggaa
gctgggctcaacattgggtttggtaagggaatctgccatgaatcagagat
tctggagatggcttctacgcatggagttatcgtgaaggaaggatctgggt
attggatcaacggcgacttcctgcctgtcaaggaggaagccgagtagttc
ctacttgagaacgaagctgtggcatacgacatctg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE586128
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||