GrainGenes Sequence Report: BE591601
Sequence
BE591601
Contig
Ta.2016.1.S1_at NSFT03P2_Contig14731
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC285142
wEST map position
BE591601
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AS 1BS 1DS
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1659-1662_L01_L01ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1659-1662_L01_L01, mRNA sequence.
Other Name
WHE1659-1662_L01_L01ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1659-1662_L01_L01
Probe
WHE1659-1662_L01_L01
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.2016.1.S1_at 1031 WHE2AFFY Ta.2016.1.S1_at 783 WHE2AFFY Ta.2016.2.S1_at 428 WHE2AFFY Ta.2016.2.S1_at 60 WHE2AFFY
DNA
aggcattcggcacgaggggcaagttctccatcaactttggtattcgcctt
ctgatgatagcaagatatggtgtgcagttggagaagggttcatttgagaa
gcggacagcagatttcttgtggatgatgatatttggtgcaacctcgttac
tggtactgtctgctattccctatctcgagacttatttgttgggtattcct
atggttagcatgcttctttatgtctggagccgagagtacccaaactctca
gataagcatgtatggcctcgtccaattgaggtcattttatctaccatggg
ctatgcttggattggatgtgatatttggctctccaatactgcctggactt
ttgggcattatggttggacatctgtactacttctttacagtgttgtatcc
gctagctagcggaaagaactacctcaagactcctatgtgggtacacaaga
ttgttgctcgattcaggttgggcgtgcaggcaaactctccagtccggcca
gctaacacaggccctactgccttcagaggaagaggctatagactcaatca
gtagactgctgcgacgcctggcgatgtacaagaggcatgaacaggttaac
atggattcaatctaaactctgtggcaactctaggaatctgggttggga
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE591601
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||