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GrainGenes Sequence Report: BE591682

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Sequence
BE591682
Contig
NSFT03P2_Contig15340
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC344578
wEST map position
BE591682
NCBI UniGene
Ta.48214
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001054466.1 Os05g0115700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AS
1DS
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1659-1662_N02_N02ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1659-1662_N02_N02, mRNA sequence.
Other Name
WHE1659-1662_N02_N02ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1659-1662_N02_N02
Probe
WHE1659-1662_N02_N02
BE591682
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gggctgctcattgttcattctatacccggcatcaatggatgttagtccac
ttaataaaggcagagaaagtactgtttggggtgtttcactcatgcttggc
tatcttggatttgatggtttcacaagcaccttccaagacaaactcttcaa
agggtatgagatggaaattcacaaccaaatattctacacgacaatgtgct
cttgtgtgattagcttgagtgggcttattctccaaaatcatcttcttcca
gctgtggactttatggttcgtcatccaaattgcttctatgatgtcctaat
cctatccactgttgcaacagctagccagttcttcatatcctacactattc
gaacattcggggc

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