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GrainGenes Sequence Report: NSFT03P2_Contig12591

Sequence
BE591974
Contig
NSFT03P2_Contig12591
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC305587
wEST map position
BE591974
NCBI UniGene
Ta.54904
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001066970.1 Os12g0548200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AS
5BS
5DS
Clone Library
Wheat heat stressed flag leaf cDNA library
Tissue
Flag leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1651-1654_A09_A09ZS Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1651-1654_A09_A09, mRNA sequence.
Other Name
WHE1651-1654_A09_A09ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA031E1X
Clone
WHE1651-1654_A09_A09
Probe
WHE1651-1654_A09_A09
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.108134.1.S1_at404WHE2AFFY
TaAffx.108134.1.S1_at157WHE2AFFY
TaAffx.27595.1.S1_at230WHE2AFFY
TaAffx.27595.1.S1_at212WHE2AFFY
TaAffx.41081.1.S1_at62WHE2AFFY
DNA
tgcagtggatgctacagaccgagctgatagggctgctgctgaagctaagg
agaaggctgctgctcaaaccagggagagggctacagcagaaagagctgct
gttgagagagtgcgacaagatgcaaagagaagagctgaccgagcagcagt
ggaaaaggctgcagctgaggttcgggaaaggcaagctgctgaggctcgag
agaggcacgctgcttctgcagcggcagcagcggcaagagaaaaacagagt
aaaccagatgaccttgagtccttttttggtatgggtgcccgagcaaacag
tgcacctaagcaaagggctccaacagtggatcctacgtttaatgctcaaa
gtcaaagtagagcgtcagcaacctctgcttcagcatcatctatgcggaag
gcatcatctacaacaaattttacggatgacctatccgcgatatttggagg
agctcccgtgccatctgatg

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