GrainGenes Sequence Report: BE601861

HVSMEh0099M19f Hordeum vulgare 5-45 DAP spike EST library HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare cDNA clone HVSMEh0099M19f, mRNA sequence.

Strain

lab_host SOLR

vulgare

Clone

HVSMEh0099M19f

Probe

[Bcl298ct652cn2647]

{SpCl-324}

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 338; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence start: 20; High quality sequence stop: 520.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Plants were grown in the greenhouse at the University of California, Riverside (Fenton, SJ Close, TJ Close). Whole spikes with awns trimmed were collected at 5, 10, 15, 20, 30 and 45 DAP (Fenton). Total RNA was prepared from each pool, equal quantities of all six RNA pools were combined, poly(A) RNA was purified from the mixture, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Choi)in the TJ Close lab at the University of California, Riverside. Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

cagcgacggacggcaggatcgaagatgatcatcccggttcgctgcttcac
ctgcgggaaggtgatcgggaacaagtgggaccactacctcgatcttctcc
aggctgattacaccgagggggatgctctggatgccttgggcttggttcgc
tactgctgccgccgtatgctcatgacccatgttgaccttatcgagaagtt
gctcaactacaacaccctggagaagacggagacaagttgaagttaattga
acccatgctgcctggaaacccttgttcctttgtactacttttcaagatgg
atggtatgctgtttcttatgtcagtgcttaggaggtgcaaagtgtcaggt
agtcctgatatgatatgaagtgggtttgtccatgaatctccaaggactat
tagtgtgaccatgcgtttctactatgtaatcttcagggctattaagttaa
ccaacaaatctaatgcattgtccaggtttttcaaacttattatgctccaa
gtgttgaacatgttaaaaaaaaaa


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.