GrainGenes Sequence Report: BE606615
Sequence
BE606615
Contig
NSFT03P2_Contig16254
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC279768
NCBI UniGene
Ta.53979
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_196598.1 EMB3010 (EMBRYO DEFECTIVE 3010); structural constituent of ribosome [Arabidopsis thaliana]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0901_H11_O21ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0901_H11_O21, mRNA sequence.
Other Name
WHE0901_H11_O21ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0901_H11_O21
Probe
BE606615
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
aaccgcgccgccgccgccgctccccagcgttcgggtcgctcgccgccggc
cagctcgcgccgcaactccgccatgaagttgaacatcgcgaaccccacca
cggggtgccagaagaaggtcgagatcgatgacgaccagaagctgcggaac
ctttatgataagaggatctcccaggaggtagttggtgatcttcttggtga
ggaattcaagggttatatcttcaagatcatgggtggatgtgataagcagg
gcttcccaatgaagcaaggagtgctaacttctgggcgtgtccgccttctg
cttcacagaggcacaccttgcttccgtggttttggcaggcgtaatggtga
gcgcaggaggaagtccgtccgtggttgcattgtcagccaagacctatcag
ttatcaacttggtgattgtcaagaagggtgagaatgatct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE606615
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||