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GrainGenes Sequence Report: BE606621

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Sequence
BE606621
Contig
Ta.24114.10.S1_x_at
NSFT03P2_Contig18528
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC314676
NCBI UniGene
Ta.28482
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Pre-alpha-/beta-gliadin A-III'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0901_H05_O09ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0901_H05_O09, mRNA sequence.
Other Name
WHE0901_H05_O09ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0901_H05_O09
Probe
BE606621
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
aacaacaacaacaacaacaacaacaacaacaacaacaacaacaacaacaa
caacaacaacaacaacaacaacaacaacaacaacaaccgtcgagccaggt
ctcctaccagcagcctcagcaacaatatccatcaggccagggctcgttcc
agccatctcagcaaaacccacaggcccagggctttgtccaacctcagcaa
ctgccgcagttcgaggaaataaggaacctagcgctacagacgctaccagc
aatgtgcaatgtctacatccctccatattgctcgaccaccattgcgccat
ttggcatcatgagtactaactgagaagagaagaactctattactagatat
atgaaacaccgttttcttaaaacatggtttggtcgttgtagcggggaaaa
ataaagtgacatgcactatcatgtaagaacccgaactagactatttcaaa
cttggaaataaaagacaa

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