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GrainGenes Sequence Report: BE606698

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Sequence
BE606698
Contig
Ta.28907.1.S1_at
Ta.28907.2.S1_a_at
NSFT03P2_Contig17883
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC281236
wEST map position
BE606698
NCBI UniGene
Ta.36757
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001057837.1 Os06g0551400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AL
7AL
7BL
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0901_A02_A03ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0901_A02_A03, mRNA sequence.
Other Name
WHE0901_A02_A03ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0901_A02_A03
Probe
WHE0901_A02_A03
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.28907.1.S1_at795WHE2AFFY
[ Show all 51 ]
DNA
gccgacgcgaccggagcaatggcggcggggtaccgggcggaggacgacta
cgactacctcttcaaggtcgtcctcatcggcgactccggcgtcggcaagt
ccaacctgctctcccgcttcacccgcaacgagttcagcctcgagtccaag
tccaccatcggggtcgagttcgccacccgctccctccaggtcgacggcaa
ggtcgtcaaggcccagatttgggacaccgccggccaggaacggtaccgtg
ctattactagcgcatactaccgtggtgctgtaggagcgttgcttgtctac
gacgtcacccggcacgcaaccttcgagaatgcagagcgctggctgaagga
actgagggaccatacggatccgaacatagttgtcatgcttgttggcaaca
agtctgatctccgccatctcgtggccg

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