GrainGenes Sequence Report: BE636837

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Sequence

BE636837

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Secale cereale (rye)

Keyword

EST

Germplasm

Blanco

Species

Secale cereale

Cultivar

Blanco

Clone Library

Secale cereale anther cDNA library

Tissue

Anther

Developmental Stage

Adult plant before anthesis

Data Source

genbank	Release 135, Apr 15 2003
genbank	Downloaded 2008-2009

Title

WHE1805-1808_E19_E19ZS Secale cereale anther cDNA library Secale cereale cDNA clone WHE1805-1808_E19_E19, mRNA sequence.

Other Name

WHE1805-1808_E19_E19ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

SC024E1X

Clone

WHE1805-1808_E19_E19

Probe

SCM135

Remark

DB_xref: taxon:4550

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

cattgcaccctatagctcatcacctgctcgtatagccacagcggtttccg
tttcgcatacatacatacatacatacacacatgcgtacatacgtaggcat
gggcaccagcagcaacgcccggcggctgatggtcgtgtcgtgcctggccg
tcctggccctcgcggccggggcgcgccggccgagcgtccaccacgccgcc
gtcccaagcgctggggcgccggctcgcatcaacgggggagtagaggcgaa
ggtccgcggtttcgcgagggcggacggggcgcggttcacggtgggcggcc
ggccgttctacccgaacggcttcaacgcgtactggctcatgtacatgggg
tcgaaccccgccgaccggagcaaggtgctcgacgtgctggaccaggcgtc
ccgcctcggcgcgacggtcatcaggacgtgggccttcaacgacggcggca
gcaaccggccgctgcagatcacccccggcgtctacagcgaggacgtgttc
ctgggac


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