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GrainGenes Sequence Report: Ta.3516.1.S1_at

Sequence	BE637573
Contig	Ta.3516.1.S1_at NSFT03P2_Contig18239
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC279435
wEST map position	BE637573
NCBI UniGene	Ta.3516
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wdk9n1.pk001.m5:fis, full insert mRNA sequence'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4AL 4DS
Clone Library	Wheat 20-45 DAP spike cDNA library
Tissue	Spike and seed
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0860_C06_E12ZS Wheat 20-45 DAP spike cDNA library Triticum aestivum cDNA clone WHE0860_C06_E12, mRNA sequence.
Other Name	WHE0860_C06_E12ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA017E1X
Clone	WHE0860_C06_E12
Probe	WHE0860_C06_E12
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.3516.1.S1_at 1023 WHE2AFFY Ta.6169.1.S1_at 119 WHE2AFFY Ta.27324.1.S1_at 94 WHE2AFFY Ta.6460.1.S1_at 58 WHE2AFFY Ta.6460.1.S1_s_at 58 WHE2AFFY
DNA	tactactcgcctcggccagtcgcctccgccaccaaaaccctaaccctagc cccggagctcaggcgacgctcgcgcctccaaatggacgggggaggcacgc atcgcacgccggaggacgtgttcagggatttccgcgcgcggcgggccggc atgattaaggcgctcaccaccgatgtggagaagttctatcagcagtgcga cccagagaaagagaatctatgtctgtatggtcttcccaatgaaacatggg aagtgaacttgcctgcagaggaggttcccccagaacttccagagccggca ctgggaattaattttgctcgggatgggatggatgagaaagattggttgtc acttgttgcggtgcacagtgatgcctggttgctagcagtagccttctact ttggagcaagattcgggtttgacaaagaatccaggaagcggctttttagc atgataaacaacctccccaccatatatgaggttgtcaccggaactgcgaa gaagcaggtcaaagaaaaa

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