GrainGenes Sequence Report: BE637589
Sequence
BE637589
Contig
NSFT03P2_Contig18655
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC277239
NCBI UniGene
Ta.54206
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Pre-alpha-/beta-gliadin A-II'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat 20-45 DAP spike cDNA library
Tissue
Spike and seed
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0860_B01_C02ZS Wheat 20-45 DAP spike cDNA library Triticum aestivum cDNA clone WHE0860_B01_C02, mRNA sequence.
Other Name
WHE0860_B01_C02ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA017E1X
Clone
WHE0860_B01_C02
Probe
BE637589
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 20 DAP and seeds at 30 to 45 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
agtgctcaatacaaatccaccatgaagacctttctcatccttgccctcct
tgctattgtagcaaccaccgccacaattgcagttagagttccagtgccac
aattgcagccacaaaatccatctcagcaacaaccacaagagcaagttcca
ttggtacaacaacaacaatttccagggcagcaacaaccatttgggggaga
acagccatatccgcagccgcgaccatttccattacaacaaccatatctgc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE637589
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||