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GrainGenes Sequence Report: BE637808

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Sequence
BE637808
Contig
Ta.11479.1.S1_at
NSFT03P2_Contig7809
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC331759
wEST map position
BE637808
NCBI UniGene
Ta.11479
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001051149.1 Os03g0728800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4DS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1755-1758_D21_D21ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1755-1758_D21_D21, mRNA sequence.
Other Name
WHE1755-1758_D21_D21ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE1755-1758_D21_D21
Probe
WHE1755-1758_D21_D21
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.11479.1.S1_at815WHE2AFFY
Ta.11479.1.S1_at745WHE2AFFY
DNA
cacatgttgcaagatgttgaaaaaaagataccaactgcattttacctttc
ccattactcacaaaaaatgctgtcgagtccctacgatatagggctcacga
tgttgctaggagtgtgacaagccgtgatatacaggaagcgcgtcgtcaag
acgtaaagaatgaaatcctcaattctgagaagttgaaagctcattttgaa
gagaatccaacagacctcgatttgctcaaacatgataagttgcttaccaa
caaatcaattcctgctcaccttcgggatgtccctgaatacctaattgacc
ccacaacgaaagaagccagcaatgcagtcaaactttcgagggctgctatg
ggtcttgataatccccgaaggaagaaaagaatgggattcaggagtgggtc
aggcaaaagtagcgatcccctcagaacgttctctgccgagggcaaatcac
gaaaac

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