GrainGenes Sequence Report: BE638008
Sequence
BE638008
Contig
Ta.3819.1.S1_at
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC362448
wEST map position
BE638008
NCBI UniGene
Ta.3819
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2AS 2DS 5AL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0995-0998_M17_M17ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0995-0998_M17_M17, mRNA sequence.
Other Name
WHE0995-0998_M17_M17ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE0995-0998_M17_M17
Probe
WHE0995-0998_M17_M17
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.3819.1.S1_at 359 WHE2AFFY Ta.3819.1.S1_at 66 WHE2AFFY TaAffx.38218.1.S1_at 100 WHE2AFFY TaAffx.38218.1.S1_at 64 WHE2AFFY
DNA
gtaccaccggtgcccatcaagaaagatagaaatcgacaaaacaagatcga
tcgatggccggtgcgcgcaaggaggctagtgttgtcgtcgccgtcaccct
cgtggtggtggtggtggccgcatcatccatgccggtggtcaagggggatg
acaagtctgcttgcgtcggcgagtactccaagctctgcggcaacgaggac
cccgcgtgcaccaccatggtcgagacgacctgccacgatgatgccgacag
cgtcaaggccatcaagggcatgttctacgagctggccaagg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE638008
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