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GrainGenes Sequence Report: BF145792

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Sequence
BF145792
Contig
NSFT03P2_Contig5360
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC4219
wEST map position
BF145792
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Germplasm
Blanco
Species
Secale cereale
Cultivar
Blanco
Chromosome
6BL
Clone Library
Secale cereale anther cDNA library
Tissue
Anther
Developmental Stage
Adult plant before anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankDownloaded 2008-2009
Title
WHE1840_G02_N04ZS Secale cereale anther cDNA library Secale cereale cDNA clone WHE1840_G02_N04, mRNA sequence.
Other Name
WHE1840_G02_N04ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
SC024E1X
Clone
WHE1840_G02_N04
Probe
WHE1840_G02_N04
Remark
DB_xref: taxon:4550
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.131747.1.S1_x_at567WHE2AFFY
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DNA
aaacagcagcactagtagatttcgagtgacaagttcagcgcaagatgcga
ccaccaggacacggcaccggcgagaagaagggcatcgtggagaacatcaa
ggaaaagctccccggtggccaaggtgaacaccagcagaccgctggcaccc
acgggcagcatggacacactggaatgacaggcacggagatgcatgatacc
acggccaccggcggcatccatgggcagcaggggcatgccggaacgactgg
cactgggacacacggcaccgccgggaccggcgagaagaagagcctcgtgg
acaaggttaaggagaagctgcccggacagcactaagcccggtgtgcccac
ggccgctacctttgcagaataaaatactccaccgtatatgaattgatcga
gtctagttcacctagctcacttggccgttggaggagcgaatgtatcttta
at

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