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GrainGenes Sequence Report: BF200530

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Sequence
BF200530
Contig
NSFT03P2_Contig15243
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BF200530
NCBI UniGene
Ta.25640
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001062347.1 Os08g0533700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6BS
6DS
7DS
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0825-0828_F05_F05ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0825-0828_F05_F05, mRNA sequence.
Other Name
WHE0825-0828_F05_F05ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA016E1X
Clone
WHE0825-0828_F05_F05
Probe
WHE0825-0828_F05_F05
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.1238.3.S1_a_at535WHE2AFFY
Ta.1238.3.S1_a_at133WHE2AFFY
Ta.1238.3.S1_a_at482WHE2AFFY
TaAffx.106385.1.S1_at141WHE2AFFY
DNA
gcgtacccccgctcacttcccgttcgttgaggcagcagggcagccacttc
ctgctcgagctcgacacgcttccctcgtgggctctcagtcggacggcgac
cctcaagctccacacccctgtggccctgtaaccctcaagctcgacgccca
gatcccctgcggccaagcccttctacccgtgagtcaagtcccagtccggc
tgctctgcttcccagctgctccttcaatccggcttcggcagctcacttct
caccacggttcagcacctcaacaaggtattagttagtcatggtgttcagc
aaggtggaagttaacttgagaaggctgcttgaggcggccccccgccagca
gaatcaggctaagcttgtccattacataaccacagccagggagctgttgg
agcaacttggagctgaaattacaccagaaggaatatcaagctagcatttt
gttgaggatccatagttacagaaagaaatacagccggtatgtgacaagat
cgtaaccatcaagtg

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