GrainGenes Sequence Report: Ta.27744.1.S1_x_at
Sequence
BF200656
Contig
Ta.27744.1.S1_x_at Ta.27744.2.S1_a_at NSFT03P2_Contig17912
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC293609
wEST map position
BF200656
NCBI UniGene
Ta.27744
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001066032.1 Os12g0123500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4BL 4DL 5AL
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0825-0828_P10_P10ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0825-0828_P10_P10, mRNA sequence.
Other Name
WHE0825-0828_P10_P10ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA016E1X
Clone
WHE0825-0828_P10_P10
Probe
WHE0825-0828_P10_P10
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.27744.2.S1_a_at 341 WHE2AFFY Ta.27744.1.S1_x_at 327 WHE2AFFY Ta.3304.1.S1_at 68 WHE2AFFY TaAffx.113232.1.S1_at 50 WHE2AFFY
DNA
accatgcctcgggcgtcctcctcttcctcctctggccttctcgttcgccg
gctccgcctttgcggccgacgcggcggtgctcggccggaaggccggtatt
ggcgccgttgccgaggaggagcccgagctgaagaaagcggcgcccgggaa
gtacgcggtgatcttcgacgcgggcagcacgggcacgcgcgtccacgtct
tccagttcgaccagaagatggagctcatcgacattggcgacggcga
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.27744.1.S1_x_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||