GrainGenes Sequence Report: BF200819

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Sequence

BF200819

Contig

Ta.3455.1.S1_at

NSFT03P2_Contig9145

Tracefile

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External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, strongly similar to NP_001045858.1 Os02g0141300 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Germplasm

Chinese Spring

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat vernalized crown cDNA library

Tissue

Crown tissue of seedling

Developmental Stage

Five-week old seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0821-0824_N11_N11ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0821-0824_N11_N11, mRNA sequence.

Other Name

WHE0821-0824_N11_N11ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

TA016E1X

Clone

WHE0821-0824_N11_N11

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

gaaatcggtgttcgcccagcacccgcgtctcgtcggattaatggaagtgc
agaatcatcttttgaggacttcgagattctccatggagacatgcaaggat
taaccgatactatggctttcctgacaagtttatctgggcttgttgggggc
gagtccgagatccctgagaagcaatcccgagaaaggactgctgcttctgt
actctttgacttggaggaggatatatatgttgcaagagcacctggacgat
tggatgtcatgggtggcattgcagattattcaggaagtcttgtcctacag
atgccccttcgagaggcctgt


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