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GrainGenes Sequence Report: BF202635

Sequence	BF202635
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC292602
wEST map position	BF202635
NCBI UniGene	Ta.6660
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_175911.1 DEAD box RNA helicase, putative (RH20) [Arabidopsis thaliana]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	3BS
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1783_D02_G03ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1783_D02_G03, mRNA sequence.
Other Name	WHE1783_D02_G03ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA019E1X
Clone	WHE1783_D02_G03
Probe	WHE1783_D02_G03
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.24586.2.S1_a_at 66 WHE2AFFY Ta.24586.2.S1_at 66 WHE2AFFY
DNA	acctacccctatccagtcacaaggttggccaatggcactgaaggggcgtg atcttattggcattgctgaaacaggatctggaaaaacacttgcttacctt ttacctgccattgttcatgttaatgctcagcctattctagctcctggtga tggtccgattgttcttgtgttagcccctacacgggaacttgctgtccaga tacagcaggagacaacaaaatttggtgcctcatcaaagataaaaagcaca tgcatatatggtggtgtcccgaaaggtcctcaagttcgtgatcttcagaa aggtgttgaaattattatagccacgccaggaagactaattgatatgatgg aatcacatcacacaaatttgcggagggtcacataccttgttttagacgag gcagatcgaatgctagacatgggttttgaacctcagattaagaaaattgt ttctcagattcgtccagatcgacaaacactttattggagtgctacctggc caaaggaagttgagctcctagcaaggactttcctttttgatccatacaag gttataattggttctgaagaactgaaagctaatcatgctatttgtcagca tgtagagatattatctgagagtcagaagtataacaagttggtcaatctac tggaggatataatggatggcagccgaattttagtattcatggaca

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