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GrainGenes Sequence Report: BF202800

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Sequence
BF202800
Contig
Ta.11646.1.A1_at
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC306245
wEST map position
BF202800
NCBI UniGene
Ta.11646
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001054150.1 Os04g0661700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2BL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1784_G04_M08ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1784_G04_M08, mRNA sequence.
Other Name
WHE1784_G04_M08ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE1784_G04_M08
Probe
CFE291
WHE1784_G04_M08
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.11646.1.A1_at728WHE2AFFY
Ta.11646.1.A1_at56WHE2AFFY
DNA
aaaacctcttccggccatcattactgaaaataataataataataatgcgg
agagcactcacaggcattttgaaaggagcagtcatgagaaagatcttaaa
gaggtgaaggttgtcaatggaaaagactctgagccttctaaaactgacaa
gtttagagatggtgacaagcatagcatgcctgaaagtattgataaaaagg
gcacctacaaagaggaaaggggttctggcaggtatgcaaaacatggattg
gatagatgggatgatgccaaaagagatcgaaaggaccttccaaaggatgc
aagagagagaaagagggattctgctgatagaagagacgtaggaaaagatg
ggaatgacgataggtcaaggcaaattactaagagctcagctagccacagc
agtagaaggtcacggtcacggtcaccaagtgggagaagccgtactatgaa
tgagagttcttcacgtgttc

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