GrainGenes Sequence Report: BF257693
Sequence
BF257693
External Databases
Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene
Hv.29283
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, weakly similar to NP_001064458.1 Os10g0370500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Morex
Clone Library
Hordeum vulgare seedling root EST library HVcDNA0007 (Etiolated and unstressed)
Tissue
Seedling root
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HVSMEf0013J20f Hordeum vulgare seedling root EST library HVcDNA0007 (Etiolated and unstressed) Hordeum vulgare subsp. vulgare cDNA clone HVSMEf0013J20f, mRNA sequence.
Strain
lab_host TJC121 vulgare
Clone
HVSMEf0013J20f
Probe
kr26D0507
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: On Nov 16, 2000 this sequence version replaced gi:11186806.; Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 336; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence start: 13; High quality sequence stop: 481. Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling roots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates , Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http: Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; Seeds were surface sterilized then germinated under axenic conditions in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedling roots were then harvested, total RNA was prepared, poly(A) RNA was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids. These steps were performed in the TJ Close laboratory at the University of California, Riverside (Choi, Close, Fenton). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:
DNA
aaaaaaattctttttttttttttttttttttttttttattggcgtgaaac
aaattattggctcatgtggtgaataaagtcacaaatactacttataaatc
attaaacaaaataatcatatatattacattaataatatcttgaggtgcca
agtgctcattttttccctctcatacattctttttctccaatacttggcag
ctcttccgttcatagttgcaaattaattgcctcaagccttttttaaggag
atgtatccgctgttgatcatccactccatactccagtggttgctaacatg
cttgtacgaccagtaagcccatccaaacgtggctttcccatccacagcca
tctgagcatttgcacaccgttggaagtctttcttggaagcaccctttacc
ttccactcagccacccattcaccaacaaaagtgggagggccatctggctt
tgtcataatgctcaaattaccggcgatctgtttattaacaaaatgggtgg
tgagctacgcattggattgggaccaactggggggtcggacaccgaaggaa
tagccccccagcgaggacggtggatagtgaccgattaaaggagtggtcga
tctgggcccgagtgcagtggttatgggggggtactgccgcgtagtgtagg
tgaagctgctaaccacccataattgacctccctgtcatggcccgacaacc
ccctatggtataccctattctacttaccgcttctacgccccgcccgctcc
ccgtttttccgcgctttccccgcctttgtgaatgacatctttctttctct
ccattgtt

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GrainGenes Sequence Report: BF257693
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