GrainGenes Sequence Report: BF264414

HV_CEa0009F20f Hordeum vulgare seedling green leaf EST library HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp. vulgare cDNA clone HV_CEa0009F20f, mRNA sequence.

Strain

lab_host TJC121

vulgare

Clone

HV_CEa0009F20f

Probe

[Bcl726ct1233cn4586]

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: On Nov 17, 2000 this sequence version replaced gi:13261484.; Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 555; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 738.

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; C.I. 16155 (Mla13) plants were greenhouse grown in the R Wise lab at Iowa State University, Ames, IA; 7 day old green seedlings were challenged with isolate A27 (AvrMla13 ) of Blumeria graminis f. sp. hordei, and leaves were harvested 20 and 24 hr post-inoculation and snap frozen; uninoculated leaves were harvested 20 hr post-inoculation (Wei, Wise). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each sample pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Choi, Close). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

Note: Vector: lambdaZAP; Site_1: EcoR1; Site_2: Xho1; C.I. 16155 (Mla13) plants were greenhouse grown in the R Wise lab at Iowa State University, Ames, IA; 7 day old green seedlings were challenged with isolate A27 (AvrMla13) of Blumeria graminis f. sp. hordei, and leaves were harvested 20 and 24 hr post-inoculation and snap frozen; uninoculated leaves were harvested 20 hr post-inoculation (Wei, Wise). In the TJ Close lab at the University of California, Riverside, total RNA was prepared from each sample pool, equal quantities of all three RNA pools were combined, poly(A) RNA was purified from the mixture, one cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Choi, Close). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Oates, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

ggatgacaccagccccccgccgccgccgcctcctcctcgccggcctgccc
ccgccgcccgcggcggcacgagtcaagcgtgggcctcatcccgctcgacg
gccgccggtgaagtggagatccgtccatctgagctcctgcagtcgccgcg
ccccgaactccacacgtaatccgaagcaatccgttccagttggctctgtt
tttccgtggatttgctctggtttttgggggctcaagcgcgagctgaaccc
ccaccatgccacacgtgaacgccaaggtgtcggcggtggctgcgacgggg
atggcggccgtgcccgcggccgcggcgccgccgccgccgccgccgaggag
gtgggagggggtcgacccggcgctggagcggatggtgctgcgcgcgtgcc
tggaccaggcccccgagcgccgccgcgtgcgcgaggccttcaaggacgtg
cagctcagcatcgaccactgcctcttcaaggcacaatacagtgacattgg
aacaaaagagtcatatgagcggaactccagaggtgtggagatattctcaa
aatgttggtttccggaaaaccatcgtatgaaagcaattgtttgtctatgc
catggttatggagacacttgtacatttttccttgatgggattgctaggaa
gattgcttcagccggatatggagtatttgcattggactaccctgggtgtg
gtctttccgaaggacttcatggtacattccaagttttgatacgcttgttg
acgatgtagccgaactttttgctagatcaaagggatcctgattcagagac
ttccagctttctgttggccatctatggtggg


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