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GrainGenes Sequence Report: NSFT03P2_Contig16209

Sequence
BF293714
Contig
Ta.6869.1.S1_at
NSFT03P2_Contig16209
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Triticum turgidum
Keyword
EST
Germplasm
Langdon-16
Species
Triticum turgidum
Triticum turgidum ssp. durum
Cultivar
Langdon-16
Clone Library
Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library
Tissue
All tissues
Developmental Stage
Different growth stages
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2154_B06_D12ZS Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library Triticum turgidum cDNA clone WHE2154_B06_D12, mRNA sequence.
Other Name
WHE2154_B06_D12ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TT039E1X
Clone
WHE2154_B06_D12
Remark
DB_xref: taxon:4571
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at North Dakota State University (Kianian, Otto, Simons). Tissues collected from seven-day etiolated seedling leaf, stem, root and seed; leaf from plant at fourth leaf stage; spike from pre-anthesis through 20 days after anthesis; flag leaf; leaf and stem tissue from tillers. and root. Total RNA and poly(A) RNA were prepared from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.6869.1.S1_at967WHE2AFFY
Ta.242.1.S1_at385WHE2AFFY
DNA
tgctgacgcagttcaactctgcgtcgctcaaccgccacctctcgagggcc
tacgggaacaacattggtggatacaagaatgatggctttgtcgaagttct
cgctgcgcagcagagcccggagaatcctaactggtttcagggtaccgcag
atgccgtgcggcagtacttatggctgtttgaggaacataatgttatggag
ttccttattctggctggagatcacctgtaccgtatggactaccaaaaatt
cattcaagcacacagagaaacagatgctgatattactgtggctgccctgc
caatggatgaggagcgcgcaactgcatttggcctgatgaaaattgacgat
gaagggagaattgttgagttttcagaaaaaccaaaaggagagaagttgaa
ggcaatgatggttgacaccaccatactgggccttgattctgagagggcca
aggaattaccttatattgctagcatgggaatctatgtttttagcaaagat
gcgatgcttcagct

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