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GrainGenes Sequence Report: TaAffx.90564.1.S1_at

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Sequence
BF428617
Contig
TaAffx.90564.1.S1_at
NSFT03P2_Contig13238
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC325135
NCBI UniGene
Ta.54980
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_200894.1 AtRABA1f (Arabidopsis Rab GTPase homolog A1f); GTP binding [Arabidopsis thaliana]'
Keyword
EST
Germplasm
TAM W-101
Species
Triticum aestivum
Cultivar
TAM W101
TAM W-101
Clone Library
Wheat drought stressed leaf cDNA library
Tissue
Leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1411_C01_F01ZS Wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE1411_C01_F01, mRNA sequence.
Other Name
WHE1411_C01_F01ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA027E1X
Clone
WHE1411_C01_F01
Probe
[Wcl293ct967cn1421]
{SpCl-82}
BF428617
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 65% and 78% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close), at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ggggccgtcgtcgggtcgggtcgggtcggccatggcctacagggcggagg
acgactacgactacctcttcaggtcgtgctcatcggcgactccggggtcg
gcaagtccaacctcctctcccgcttcacccgcaacgagttcagcctcgag
tccaagtccaccatcggcgtcgagttcgccaccaggagcatccgggtcga
cgacaaggtcgtcaaggcccagatctgggacacggcaggacaggagaggt
atcgggcaattacaagtgcatactatcgaggggctgttggtgcgcttgtc
gtgtacgatgtgacacgccatgtgacctttgagaatgtggagaggtggtt
gaaggacctccgggatcacac

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