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GrainGenes Sequence Report: BF473034

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Sequence	BF473034
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC305596
wEST map position	BF473034
NCBI UniGene	Ta.13573
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'LOB1'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat 5-15 DAP spike cDNA library
Tissue	Spike
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0927_D10_H19ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0927_D10_H19, mRNA sequence.
Other Name	WHE0927_D10_H19ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA018E1X
Clone	WHE0927_D10_H19
Probe	WHE0927_D10_H19
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.1590.1.S1_at 254 WHE2AFFY Ta.1590.1.S1_at 127 WHE2AFFY
DNA	gttcctgtgtcctccgtctctccgtctcccggcgaggaggagagcatgcc tctatgtccacggagagggagagactcgacgagatcggtaagaagatcaa gcgagagccggaccccgcctccgccatcgcgggcgtcgtagtcgccgtct cacagaccgagcaccacgtccctcgccgcctaggcctcgttggcccaggc atcggcggcgccgcgaacatagcgaccccgtgcgccgcgtgcaagctcct ccgccggcgctgcgcgcacgagtgccccttcgccccctacttctccccgc acgagccccacaagttcgccgccgtccacaaggtcttcggcgccagcaac gtctccaagatgctcctggaggtgcccgagccggagagagccgacgcggc gagcagcctggtgtacgaggcgaacctgcggctgggcgacccggtgtacg gctgcatgggcgcgatctccatgctgcagcagcaggtgaacgcgctggag gccgagctgcaggccgtcagggccgagatattcaagcacaggtaccggca gaccggcgtcggcgtcggcgtcggcgcggcaaacctcattgtcgacgacg acgtcagtg

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