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GrainGenes Sequence Report: Ta.365.1.S1_at

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Sequence
BF473126
Contig
Ta.365.1.S1_at
Ta.365.3.S1_a_at
NSFT03P2_Contig16094
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC286532
wEST map position
BF473126
NCBI UniGene
Ta.365
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001055553.1 Os05g0414700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5BL
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0927_H11_P21ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0927_H11_P21, mRNA sequence.
Other Name
WHE0927_H11_P21ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0927_H11_P21
Probe
WHE0927_H11_P21
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.365.1.S1_at1033WHE2AFFY
[ Show all 9 ]
DNA
acagttcacttcagaaatgaagacacttggccaagtaaggaatcgcaact
tggttcctcttcttggattttgcattgctaagaaggagaagttgttggtg
tacaagcacacacccaaaggttcactctatgatcagttacacgaagaggg
gaatgattgtaagatggattggcctctgaggttaagaattggtattggtg
cagcaaaaggtcttgcatatcttcaccacacttgcaatcctcgaatcctt
caccgcaatataagctccaaatgcattctcttggatgacgactacgaacc
aaagatttcggattttgggcttgctaggcttatgaaccccctagacaccc
atctcagcacctttgtcaatggggaatttggagatatcggttatgtggca
ccagagtacgggagcactctggtggccacgccgaagggtgacgtctacag
cttcggagtggttctccttgagcttatcaccggtgagaggcctactcagg
tttccactgctccagataacttcagggggaatctagtagagtggatcacc
tacctatccaacaattccattctcca

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