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GrainGenes Sequence Report: BF473139

Sequence	BF473139
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC312607
wEST map position	BF473139
NCBI UniGene	Ta.39291
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001064794.1 Os10g0464400 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	6AS 6BS 6DS
Clone Library	Wheat 5-15 DAP spike cDNA library
Tissue	Spike
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0922_B02_C04ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0922_B02_C04, mRNA sequence.
Other Name	WHE0922_B02_C04ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA018E1X
Clone	WHE0922_B02_C04
Probe	WHE0922_B02_C04
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	TaAffx.8051.1.S1_at 64 WHE2AFFY TaAffx.5553.1.S1_x_at 54 WHE2AFFY TaAffx.59438.1.S1_at 52 WHE2AFFY TaAffx.59438.1.S1_s_at 52 WHE2AFFY Ta.7261.1.S1_at 50 WHE2AFFY
DNA	acgcctccacccccaccccacctcaccccctcctcccgcctctcacgcgc gtcgccgttcccgttctatctcgccgggacatcgtgttcctgctgtcttc gccccgtcccagtctttggccattgaggttgggcttcccaaataatttgt ctcttgctgcatttacctcgtccgtccttggaaatggcagcaccgaagcc cattactcgccttatttcccatgtcattcttgatttggatggtaccctct taaacacagattgcattgtaagccaggtgctgaaaccatttattgttaaa aatgggaaaaagtgggatagcaagaaagctcacaaatttgtcggaaagac accttatgaagccgcagctgttgttttagaagattacggactcccttact ccaccgaagagttcctctcattgattaatccaatgttcagcgagcaatgg ggcaacataaaagctcttcctggagctaatcggttaataaaacatttgaa gagcagcagggtgccagctgctttagcttccaactcttcaagatcaaaca ttgagtccaaaatctcgtgccagcaagggtggaaagagtatttctctgca attgttggtgcagatga

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