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GrainGenes Sequence Report: BF473392

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Sequence	BF473392
Contig	NSFT03P2_Contig18621
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC308685
NCBI UniGene	Ta.54206
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Pre-alpha-/beta-gliadin A-II'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat 5-15 DAP spike cDNA library
Tissue	Spike
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0923_H09_P17ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0923_H09_P17, mRNA sequence.
Other Name	WHE0923_H09_P17ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA018E1X
Clone	WHE0923_H09_P17
Probe	BF473392
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	catatccgcagctgcaaccatttccatcacaacaaccatatatgcagctg caaccatttccgcagccgcaactaccatatccgcagccgcaactaccata tccgcagccgcaaccatttcgaccacaacaatcatatccacaaccgcaac cacagtattcgcaaccacaacaaccaatttcgcagcagcagcagcagcag cagcaacaacaacaacaacaacagatccttcaacaaattttgcaacaaca actgattccatgcagggatgttgtattgcaacaacacagcatagcgcatg gaagctcacaagttttgcaacaaagtacttaccagctggtgcaacaattg tgttgtcagcagctgtggcagatccccgagcagtcgcggtgccaagccat ccacaatgttgttcatgctattattctgcatcaacaacaacaacaacaac aacaacaaccgttgagccaggtctgcttccaacagtctcaacaacaatat ccatc

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