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GrainGenes Sequence Report: BF473436

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Sequence	BF473436
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	BF473436
DB Remark	Locus Source: Triticum aestivum (bread wheat)
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	3BL 5BL 5DL
Clone Library	Wheat 5-15 DAP spike cDNA library
Tissue	Spike
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0924_D07_H14ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0924_D07_H14, mRNA sequence.
Other Name	WHE0924_D07_H14ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA018E1X
Clone	WHE0924_D07_H14
Probe	[BF473436.1] WHE0924_D07_H14
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.10908.1.A1_at 355 WHE2AFFY Ta.10908.1.A1_at 92 WHE2AFFY
DNA	gccaccggcccctcgctccttgtcgccgctctggatggccactgctccca acatcgagatgatcgcctcttcgctgcgcagctgctccctcaacggcggc ggctggcggccgggccggcgccaccgccacggccacgccaggggcgctga gggcagcaacgacagcgaaggcgtcaccgtcgagctcaactccgacgtcg ccctgccctaccactggcagcagtgcctcgacatccggacggggcaagtg tactacatcaactgggaggacggcacccggacgactatcgaccctcgcac gccgtcgctttgctccgccttgtcgacgccgcggtccacctgtttcactt cacaccgcgcagccttcacttcgtcgtccagctctgggtacacctccgcg gcgtccagcgtcaccggcggctacggctacggctatgatgacagcgacgg ctatagtgaaggctacggcgacgacgaagagagcagcagcagcagcagcc ggagctccggcgtctcgtccgtgctctcgtctttcttccctagtaacgag ccctcct

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