GrainGenes Sequence Report: BF473883
Sequence
BF473883
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BF473883
NCBI UniGene
Ta.10915
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AL
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0932_D08_H16ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0932_D08_H16, mRNA sequence.
Other Name
WHE0932_D08_H16ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0932_D08_H16
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
TaAffx.114925.1.S1_at 94 WHE2AFFY
[ Show all 6 ]
DNA
atctttatttgcttgttcgattcagtctggagcaggagcagctgctactc
ccgacctccgattccttctttccatggatgaccacgaagacctccgcatg
atgtgctgctgctcggggccctccctatgcttctcctcgctcgtaagggt
ccagggttagctgctcaggggccatggtgtgttccgtgtggcgctgcctt
cgcggccgccgccgttcccttgttgtgtctctgagactggaggtccctgc
tcgccaactcacagacggttgttctccgaccagttaaaaaagaattttct
gaaggaggaggtgccgaaaggggatgggagcaacaagtctgaatcgaaaa
gtcaaagaagtcgaacctgaagaagtgcagggaagaattatagttgcgtg
tggctggctggttcaggggaatttcagggtggcgcgggtgccggagcagg
ctcctaggcactggcgatctgtcggacgga
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BF473883
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||