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GrainGenes Sequence Report: Ta.10785.1.S1_at

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Sequence
BF473884
Contig
Ta.10785.1.S1_at
NSFT03P2_Contig6442
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BF473884
NCBI UniGene
Ta.48513
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001056857.1 Os06g0156900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7AS
7DS
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0932_D09_H18ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0932_D09_H18, mRNA sequence.
Other Name
WHE0932_D09_H18ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0932_D09_H18
Probe
WHE0932_D09_H18
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.10785.1.S1_at1261WHE2AFFY
[ Show all 6 ]
DNA
gactctccttgcttttggccaaggaacgtacacatacacaaacatacatt
ggatgcatgaagaaggggcctgtttttactgatcccaaattgaaatggta
cgagccacagtccttcctacttggatcagaatacttccttcatgcatatg
ggcctatttatgctttatcagctgatgtggtggcaagcttggttgctgtg
agaaacaacagtttccggatgttcaacaacgaggatgttactattggatc
ctggatgctcgctatgaacgtcaaccacgagaacacgcacgctctcaccg
aacccgagtgcacagcgtcctcaattgctgtctgggacattccaaaatgc
tcagggctatgccacccggaggtaaagatgctagagcttcaccagcggaa
agagtgcacgggtggtccaacggaggaccaggcggcatgcatggacactg
gaccaggcggcatgtcatgatgacacaaacggtcggttgacccaatagat
tcttggagtgcctgcacatctgattcgatccgaacatcaggtagcacaat
cattgccgttttgcaagcataccaccaagcacgctgacgaagaaccctac
cgctgcgtgcggttcaggcgccacaccacgcagctgcaagcacattctta
c

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