GrainGenes Sequence Report: BF473914
Sequence
BF473914
Contig
Ta.6548.1.S1_a_at Ta.6548.2.S1_a_at NSFT03P2_Contig8755
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC289830
NCBI UniGene
Ta.6548
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001045485.1 Os01g0963300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0932_G05_N10ZS Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0932_G05_N10, mRNA sequence.
Other Name
WHE0932_G05_N10ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA018E1X
Clone
WHE0932_G05_N10
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.6548.1.S1_a_at 880 WHE2AFFY
[ Show all 18 ]
DNA
tccgccgcacggcgcccacaaatcctagtagggttcgttcgccgccggga
gaggacggccggccggtcgaatcccagcctccccccaccccacccgcgga
tccggcctccgccatgagctccgcgcaggaccccttctacatcgtccggg
aggagatccagggctcgattggtaagctgcagactactttccatcggtgg
gagcaagtttcttcaaacactggagaatatgttcatataacaaaagagct
tctcaccagctgtgaaagcatcgagtggcaggtggatgagttagaaaaga
caatttcagttgcatcaagggatccagcgtattatggactcgatgaagtt
gaactctccagacgacggaactggactggctctgctcgtaatcagatcgg
tactgtcaaaagagctgttgaaaaggggaagagcaatcctgcaatggcaa
gacatcaggacaatggcacaagcagaactaactactattcttcccaagac
aatgatgactatattgcttcagaatcagatagacagcttctactcatg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BF473914
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||